Guest imported_Tesseract Posted February 5, 2003 Share Posted February 5, 2003 Tesser: did you know that lizards grow a new tail if it gets cut out? fr8o: really? fr8o: dude, that's rad fr8o: i want that on my car Tesser: man, if i was lizard i'd find the perfect dose of tail, and i'd just sit on my ass eating my tail like theres no tommorow Tesser: Dope dna mix: VW/lizard fr8o: hahahahahahahahaahahahaahah fr8o: holler, holler. Link to comment Share on other sites More sharing options...
NAYM IS DEAD Posted February 5, 2003 Share Posted February 5, 2003 aww man i thought this was going to be about the DNA SXE crew from syracuse...id talk shit about them but theyre all really big... Link to comment Share on other sites More sharing options...
RumPuncher Posted February 5, 2003 Share Posted February 5, 2003 Originally posted by Tesseract and i'd just sit on my ass eating my tail like theres no tommorow sit on your ass and eat your tail? Is it possible to have an ass and a tail ? you've got some crazed DNA son! Link to comment Share on other sites More sharing options...
Guest imported_Tesseract Posted February 5, 2003 Share Posted February 5, 2003 Re: Re: DNA stories Originally posted by Kilo7- sit on your ass and eat your tail? Is it possible to have an ass and a tail ? you've got some crazed DNA son! off course its posible, lizards have both...you want a sketch? Link to comment Share on other sites More sharing options...
RumPuncher Posted February 5, 2003 Share Posted February 5, 2003 YES. I do need a sketch as it's hard to visualize. http://www.nevadawilderness.org/wilderness/lizard_tail.jpg'> ^ I dont see ass ! Link to comment Share on other sites More sharing options...
Guest imported_Tesseract Posted February 5, 2003 Share Posted February 5, 2003 even though it was meant metaphorical for the most part here it goes: the lizards ass(butocks is better) is cover by the tail....thats why you dont see it) A lizard thats sitting on its ass and eats its own tail looks like a sitted man that tries to suck his own dick...only its a lizard... bon apetite Link to comment Share on other sites More sharing options...
RumPuncher Posted February 5, 2003 Share Posted February 5, 2003 Originally posted by Tesseract the lizards ass(butocks is better) is cover by the tail....thats why you dont see it well... you'd have to remove the tail before you get to the ass, hence making it impossible to sit on the ass and eat the tail. chicken vs. egg for the 2k3 ! Link to comment Share on other sites More sharing options...
Guest imported_Tesseract Posted February 5, 2003 Share Posted February 5, 2003 Man...cant a lizard sit on her tail AND ass?...jeez Link to comment Share on other sites More sharing options...
!@#$% Posted February 5, 2003 Share Posted February 5, 2003 i've got a gazillion DNA stories.. just today i digested some DNA with restriction enzymes, ran them on a 1% agarose gel and phenol-chloroform purified the samples.. next i will be ligating the purified DNA insert to a variety of different vectors in varied concentrations, then tomorrow those ligations will be transformed into DH5-alpha E.coli cells. this will be used in a multitude of PCR protocols, including site-directed mutagenesis, direct sequencing, and transfection :king: Link to comment Share on other sites More sharing options...
RumPuncher Posted February 5, 2003 Share Posted February 5, 2003 ^ very cool! but can it make a decent cup of coffee ? Link to comment Share on other sites More sharing options...
!@#$% Posted February 5, 2003 Share Posted February 5, 2003 i can make a decent cup of anything..;) ;) Link to comment Share on other sites More sharing options...
doctor unk Posted February 6, 2003 Share Posted February 6, 2003 DNA?...in syracuse? Originally posted by NAYM IS DEAD aww man i thought this was going to be about the DNA SXE crew from syracuse...id talk shit about them but theyre all really big... theres another DNA crew...whatll take to get those guys to stop writing that......and....hahaha.......what does it stand for for them....hahaha i have a lizard... Link to comment Share on other sites More sharing options...
S@T@N Posted February 6, 2003 Share Posted February 6, 2003 I learned how to excel in biological warfare by making super germs. We took some E. Coli samples on an agarose gel like !@#$% did, but ours had a mixture of Kanamycin (antibiotic). After letting the culture sit for a day, we came back and found what we expected: 99% of the bacteria were gone. There were, however, a few small colonies of bacteria remaining. These bacteria had a gene for resistance to kanamycin. We also did the same experiment with a group of bacteria subjected to ampicillin in their agar. The surviving bacteria in both batches were then placed in pipettes. Using DNA restriction enzymes we cut the 2 bacteria's chromosomes in strategic places that would allow the genes for antibiotic resistance to float freely. Then, using DNA Ligase, we attempted to reattach those chromosomes to make a gene with resistance to both antibiotics. We then took a fresh batch of E. Coli that had been on a 'feeding' pattern and introduced them to the engineered chromosomes at a point in the day when they would be most active, hoping they would take in said chromosomes. These bacteria were then put on a petri dish with an agarose gel that contained BOTH kanamycin and ampicillin, and allowed to grow overnight. The bacteria that thrived on the plate the next day had resistance to both of the used antibiotics. Using this method I could easily create an unstoppable strain of the bacteria responsible for strep throat, bronchitis, etc. The world is at my mercy.... Muhahahhaha. Link to comment Share on other sites More sharing options...
NAYM IS DEAD Posted February 6, 2003 Share Posted February 6, 2003 Re: DNA?...in syracuse? Originally posted by doctor unk theres another DNA crew...whatll take to get those guys to stop writing that......and....hahaha.......what does it stand for for them....hahaha i have a lizard... it has nothing to do with graff at all..its a stupid straight edge crew...but i wouldnt talk shit because well...they beat up the cromags...not kidding...if anyone was at hellfest they mightve seen a little scuffle between DNA and harley flannigan that was started when earth crisis beat the shit out of the cro mags in europe...those guys arent to be fucked with... Link to comment Share on other sites More sharing options...
Kr430n5_666 Posted February 6, 2003 Share Posted February 6, 2003 http://www.metal-reviews.com/images/btg.gif'> Link to comment Share on other sites More sharing options...
Guest spec Posted February 6, 2003 Share Posted February 6, 2003 http://www.richmangalleries.com/images/No.-6-Train-Uptown.jpg'> wake me when we get to bklyn Link to comment Share on other sites More sharing options...
Kr430n5_666 Posted February 6, 2003 Share Posted February 6, 2003 http://www.metal-reviews.com/images/btgrunge.gif'> Link to comment Share on other sites More sharing options...
Guest spec Posted February 6, 2003 Share Posted February 6, 2003 http://www.users.bigpond.com/finehoned/Images/Ray-Ilustrisimo-Train-6.jpg'> :crazy: Link to comment Share on other sites More sharing options...
!@#$% Posted February 6, 2003 Share Posted February 6, 2003 Originally posted by S@T@N I learned how to excel in biological warfare by making super germs. We took some E. Coli samples on an agarose gel like !@#$% did, but ours had a mixture of Kanamycin (antibiotic). After letting the culture sit for a day, we came back and found what we expected: 99% of the bacteria were gone. There were, however, a few small colonies of bacteria remaining. These bacteria had a gene for resistance to kanamycin. We also did the same experiment with a group of bacteria subjected to ampicillin in their agar. The surviving bacteria in both batches were then placed in pipettes. Using DNA restriction enzymes we cut the 2 bacteria's chromosomes in strategic places that would allow the genes for antibiotic resistance to float freely. Then, using DNA Ligase, we attempted to reattach those chromosomes to make a gene with resistance to both antibiotics. We then took a fresh batch of E. Coli that had been on a 'feeding' pattern and introduced them to the engineered chromosomes at a point in the day when they would be most active, hoping they would take in said chromosomes. These bacteria were then put on a petri dish with an agarose gel that contained BOTH kanamycin and ampicillin, and allowed to grow overnight. The bacteria that thrived on the plate the next day had resistance to both of the used antibiotics. Using this method I could easily create an unstoppable strain of the bacteria responsible for strep throat, bronchitis, etc. The world is at my mercy.... Muhahahhaha. ooooooooohhh so close.. an agarose gel is a separation method for determining the size of DNA fragments... not a media for bacteria growth. you're thinking of agar (as you mentioned in the protocol)...and an agar medium but yeah, you could still create those super-resistant strains..if you know where to find the viruses. Link to comment Share on other sites More sharing options...
Cracked Ass Posted February 6, 2003 Share Posted February 6, 2003 Heh...you have access to chloroform... Kidnap those bike-lock-wielding fools and get medieval on em. How's your head? :beat: Link to comment Share on other sites More sharing options...
!@#$% Posted February 6, 2003 Share Posted February 6, 2003 healing nicely i'm trying to keep from scratching the scab..disgusting i know, but you know how it gets itchy and it feels bizarre to have some hard crusty thing on your body? eeewwwwww. almost gone.. i haven't gotten to look at my flicks yet. i moved and now i can't find my camera.. yeah, the ol' chloroform on the rag trick.. it's ridiculous, about 5 feet away from me right now there is a liter of chloroform.. i'm fuckin eyeballin that shyte right now!! gas those fucks!!! lol... dumb fuckin kids. they live in a city where last year, 1/4 of the homicide victims were under the age of 18.. i give half of em another 5 years..tops. Link to comment Share on other sites More sharing options...
fr8oholic Posted February 6, 2003 Share Posted February 6, 2003 HOLLER. just saw this. i had a primo AIM conversation get nixed when the site got reloaded. it's probably better left unheard. Link to comment Share on other sites More sharing options...
Guest beardo Posted February 6, 2003 Share Posted February 6, 2003 i've got it, fr8oh.. .. . i dont have your email handy, holler if you want it Link to comment Share on other sites More sharing options...
fr8oholic Posted February 6, 2003 Share Posted February 6, 2003 OH LORD. Link to comment Share on other sites More sharing options...
S@T@N Posted February 6, 2003 Share Posted February 6, 2003 Originally posted by !@#$% ooooooooohhh so close.. an agarose gel is a separation method for determining the size of DNA fragments... not a media for bacteria growth. you're thinking of agar (as you mentioned in the protocol)...and an agar medium but yeah, you could still create those super-resistant strains..if you know where to find the viruses. Yup, you're right, that's gel electrophoresis, and it IS agar that you use on the plates. Of course, if I hadn't taken my first year of college biology in my junior year of high school I could probably remember that. Link to comment Share on other sites More sharing options...
!@#$% Posted February 6, 2003 Share Posted February 6, 2003 ok smarty pants. i'll forgive you ;) Link to comment Share on other sites More sharing options...
Recommended Posts
Archived
This topic is now archived and is closed to further replies.