Jump to content
Search In
  • More options...
Find results that contain...
Find results in...
Sign in to follow this  
Guest imported_Tesseract

DNA stories

Recommended Posts

Guest imported_Tesseract

Tesser: did you know that lizards grow a new tail if it gets cut out?

fr8o: really?

fr8o: dude, that's rad

fr8o: i want that on my car

Tesser: man, if i was lizard i'd find the perfect dose of tail, and i'd just sit on my ass eating my tail like theres no tommorow

Tesser: Dope dna mix: VW/lizard

fr8o: hahahahahahahahaahahahaahah

fr8o: holler, holler.

Share this post


Link to post
Share on other sites
Originally posted by Tesseract

and i'd just sit on my ass eating my tail like theres no tommorow

 

sit on your ass and eat your tail?

 

Is it possible to have an ass and a tail ?

 

you've got some crazed DNA son!

Share this post


Link to post
Share on other sites
Guest imported_Tesseract

Re: Re: DNA stories

 

Originally posted by Kilo7-

sit on your ass and eat your tail?

 

Is it possible to have an ass and a tail ?

 

you've got some crazed DNA son!

 

 

off course its posible, lizards have both...you want a sketch?

Share this post


Link to post
Share on other sites
Guest imported_Tesseract

even though it was meant metaphorical for the most part here it goes:

 

 

the lizards ass(butocks is better) is cover by the tail....thats why you dont see it)

 

 

A lizard thats sitting on its ass and eats its own tail looks like a sitted man that tries to suck his own dick...only its a lizard...

 

bon apetite

Share this post


Link to post
Share on other sites
Originally posted by Tesseract

the lizards ass(butocks is better) is cover by the tail....thats why you dont see it

 

well...

you'd have to remove the tail before you get to the ass, hence making it impossible to sit on the ass and eat the tail.

 

chicken vs. egg for the 2k3 !

Share this post


Link to post
Share on other sites
Guest imported_Tesseract

Man...cant a lizard sit on her tail AND ass?...jeez

Share this post


Link to post
Share on other sites

i've got a gazillion DNA stories..

 

just today i digested some DNA with restriction enzymes, ran them on a 1% agarose gel and phenol-chloroform purified the samples..

 

next i will be ligating the purified DNA insert to a variety of different vectors in varied concentrations, then tomorrow those ligations will be transformed into DH5-alpha E.coli cells.

 

this will be used in a multitude of PCR protocols, including site-directed mutagenesis, direct sequencing, and transfection

:king:

Share this post


Link to post
Share on other sites

DNA?...in syracuse?

 

Originally posted by NAYM IS DEAD

aww man i thought this was going to be about the DNA SXE crew from syracuse...id talk shit about them but theyre all really big...

theres another DNA crew...whatll take to get those guys to stop writing that......and....hahaha.......what does it stand for for them....hahaha

i have a lizard...

Share this post


Link to post
Share on other sites

I learned how to excel in biological warfare by making super germs.

 

We took some E. Coli samples on an agarose gel like !@#$% did,

but ours had a mixture of Kanamycin (antibiotic). After letting the

culture sit for a day, we came back and found what we expected:

99% of the bacteria were gone. There were, however, a few small

colonies of bacteria remaining. These bacteria had a gene for resistance

to kanamycin. We also did the same experiment with a group of bacteria

subjected to ampicillin in their agar. The surviving bacteria in both

batches were then placed in pipettes. Using DNA restriction enzymes

we cut the 2 bacteria's chromosomes in strategic places that would

allow the genes for antibiotic resistance to float freely. Then, using

DNA Ligase, we attempted to reattach those chromosomes to make

a gene with resistance to both antibiotics. We then took a fresh batch

of E. Coli that had been on a 'feeding' pattern and introduced them to

the engineered chromosomes at a point in the day when they would be

most active, hoping they would take in said chromosomes. These

bacteria were then put on a petri dish with an agarose gel that contained

BOTH kanamycin and ampicillin, and allowed to grow overnight. The

bacteria that thrived on the plate the next day had resistance to both

of the used antibiotics.

 

 

Using this method I could easily create an unstoppable strain of the

bacteria responsible for strep throat, bronchitis, etc.

 

The world is at my mercy.... Muhahahhaha.

Share this post


Link to post
Share on other sites

Re: DNA?...in syracuse?

 

Originally posted by doctor unk

theres another DNA crew...whatll take to get those guys to stop writing that......and....hahaha.......what does it stand for for them....hahaha

i have a lizard...

 

it has nothing to do with graff at all..its a stupid straight edge crew...but i wouldnt talk shit because well...they beat up the cromags...not kidding...if anyone was at hellfest they mightve seen a little scuffle between DNA and harley flannigan that was started when earth crisis beat the shit out of the cro mags in europe...those guys arent to be fucked with...

Share this post


Link to post
Share on other sites
Originally posted by S@T@N

I learned how to excel in biological warfare by making super germs.

 

We took some E. Coli samples on an agarose gel like !@#$% did,

but ours had a mixture of Kanamycin (antibiotic). After letting the

culture sit for a day, we came back and found what we expected:

99% of the bacteria were gone. There were, however, a few small

colonies of bacteria remaining. These bacteria had a gene for resistance

to kanamycin. We also did the same experiment with a group of bacteria

subjected to ampicillin in their agar. The surviving bacteria in both

batches were then placed in pipettes. Using DNA restriction enzymes

we cut the 2 bacteria's chromosomes in strategic places that would

allow the genes for antibiotic resistance to float freely. Then, using

DNA Ligase, we attempted to reattach those chromosomes to make

a gene with resistance to both antibiotics. We then took a fresh batch

of E. Coli that had been on a 'feeding' pattern and introduced them to

the engineered chromosomes at a point in the day when they would be

most active, hoping they would take in said chromosomes. These

bacteria were then put on a petri dish with an agarose gel that contained

BOTH kanamycin and ampicillin, and allowed to grow overnight. The

bacteria that thrived on the plate the next day had resistance to both

of the used antibiotics.

 

 

Using this method I could easily create an unstoppable strain of the

bacteria responsible for strep throat, bronchitis, etc.

 

The world is at my mercy.... Muhahahhaha.

 

 

ooooooooohhh

so close..

an agarose gel is a separation method for determining the size of DNA fragments...

not a media for bacteria growth.

 

you're thinking of agar (as you mentioned in the protocol)...and an agar medium

 

but yeah, you could still create those super-resistant strains..if you know where to find the viruses.

Share this post


Link to post
Share on other sites

Heh...you have access to chloroform...

Kidnap those bike-lock-wielding fools and get medieval on em.

How's your head? :beat:

Share this post


Link to post
Share on other sites

healing nicely

 

i'm trying to keep from scratching the scab..disgusting i know, but you know how it gets itchy and it feels bizarre to have some hard crusty thing on your body?

 

eeewwwwww.

almost gone..

 

i haven't gotten to look at my flicks yet.

i moved and now i can't find my camera..

 

 

yeah, the ol' chloroform on the rag trick..

it's ridiculous, about 5 feet away from me right now there is a liter of chloroform..

 

i'm fuckin eyeballin that shyte right now!!

 

gas those fucks!!!

 

lol...

 

 

dumb fuckin kids.

they live in a city where last year, 1/4 of the homicide victims were under the age of 18..

i give half of em another 5 years..tops.

Share this post


Link to post
Share on other sites

HOLLER.

 

just saw this. i had a primo AIM conversation get nixed when the site got reloaded. it's probably better left unheard.

Share this post


Link to post
Share on other sites
Guest beardo

i've got it, fr8oh.. .. .

 

i dont have your email handy, holler if you want it

Share this post


Link to post
Share on other sites
Originally posted by !@#$%

ooooooooohhh

so close..

an agarose gel is a separation method for determining the size of DNA fragments...

not a media for bacteria growth.

 

you're thinking of agar (as you mentioned in the protocol)...and an agar medium

 

but yeah, you could still create those super-resistant strains..if you know where to find the viruses.

 

Yup, you're right, that's gel electrophoresis, and it IS agar that you use

on the plates. Of course, if I hadn't taken my first year of college biology

in my junior year of high school I could probably remember that.

Share this post


Link to post
Share on other sites

Register for a 12ozProphet forum account or sign in to comment

You need to be a forum member in order to comment. Forum accounts are separate from shop accounts.

Create an account

Register to become a 12ozProphet forum member.

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
Sign in to follow this  

×